Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein.
Identifieur interne : 000041 ( Psycho/Analysis ); précédent : 000040; suivant : 000042Cell adhesion as a novel approach to determining the cellular binding motif on the severe acute respiratory syndrome coronavirus spike protein.
Auteurs : Hsin-Hou Chang [Taïwan] ; Po-Kong Chen [Taïwan] ; Guan-Ling Lin [Taïwan] ; Chun-Jen Wang [Taïwan] ; Chih-Hsien Liao [Taïwan] ; Yu-Cheng Hsiao [Taïwan] ; Jing-Hua Dong [Taïwan] ; Der-Shan Sun [Taïwan]Source :
- Journal of virological methods [ 1879-0984 ] ; 2014.
Descripteurs français
- KwdFr :
- MESH :
- isolement et purification : Antiviraux, Peptides.
- métabolisme : Glycoprotéine de spicule des coronavirus.
- physiologie : Virus du SRAS.
- Adhérence cellulaire, Animaux, Attachement viral, Humains, Liaison aux protéines, Lignée cellulaire, Sites de fixation.
English descriptors
- KwdEn :
- MESH :
- chemical , isolation & purification : Antiviral Agents, Peptides.
- chemical , metabolism : Spike Glycoprotein, Coronavirus.
- physiology : SARS Virus.
- Animals, Binding Sites, Cell Adhesion, Cell Line, Humans, Protein Binding, Virus Attachment.
Abstract
Emerging life threatening pathogens such as severe acute aspiratory syndrome-coronavirus (SARS-CoV), avian-origin influenzas H7N9, and the Middle East respiratory syndrome coronavirus (MERS-CoV) have caused a high case-fatality rate and psychological effects on society and the economy. Therefore, a simple, rapid, and safe method to investigate a therapeutic approach against these pathogens is required. In this study, a simple, quick, and safe cell adhesion inhibition assay was developed to determine the potential cellular binding site on the SARS-CoV spike protein. Various synthetic peptides covering the potential binding site helped to minimize further the binding motif to 10-25 residues. Following analyses, 2 peptides spanning the 436-445 and 437-461 amino acids of the spike protein were identified as peptide inhibitor or peptide vaccine candidates against SARS-CoV.
DOI: 10.1016/j.jviromet.2014.01.022
PubMed: 24530430
Affiliations:
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pubmed:24530430Le document en format XML
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Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan; Institute of Medical Sciences, Tzu-Chi University, Hualien</wicri:regionArea><wicri:noRegion>Hualien</wicri:noRegion></affiliation></author><author><name sortKey="Chen, Po Kong" sort="Chen, Po Kong" uniqKey="Chen P" first="Po-Kong" last="Chen">Po-Kong Chen</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Medical Sciences, Tzu-Chi University, Hualien</wicri:regionArea><wicri:noRegion>Hualien</wicri:noRegion></affiliation></author><author><name sortKey="Lin, Guan Ling" sort="Lin, Guan Ling" uniqKey="Lin G" first="Guan-Ling" last="Lin">Guan-Ling Lin</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Institute of Medical Sciences, Tzu-Chi University, Hualien</wicri:regionArea><wicri:noRegion>Hualien</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Chun Jen" sort="Wang, Chun Jen" uniqKey="Wang C" first="Chun-Jen" last="Wang">Chun-Jen Wang</name><affiliation wicri:level="1"><nlm:affiliation>The Department of Life Sciences, Tzu-Chi University, Hualien, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>The Department of Life Sciences, Tzu-Chi University, Hualien</wicri:regionArea><wicri:noRegion>Hualien</wicri:noRegion></affiliation></author><author><name sortKey="Liao, Chih Hsien" sort="Liao, Chih Hsien" uniqKey="Liao C" first="Chih-Hsien" last="Liao">Chih-Hsien Liao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien</wicri:regionArea><wicri:noRegion>Hualien</wicri:noRegion></affiliation></author><author><name sortKey="Hsiao, Yu Cheng" sort="Hsiao, Yu Cheng" uniqKey="Hsiao Y" first="Yu-Cheng" last="Hsiao">Yu-Cheng Hsiao</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien</wicri:regionArea><wicri:noRegion>Hualien</wicri:noRegion></affiliation></author><author><name sortKey="Dong, Jing Hua" sort="Dong, Jing Hua" uniqKey="Dong J" first="Jing-Hua" last="Dong">Jing-Hua Dong</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien</wicri:regionArea><wicri:noRegion>Hualien</wicri:noRegion></affiliation></author><author><name sortKey="Sun, Der Shan" sort="Sun, Der Shan" uniqKey="Sun D" first="Der-Shan" last="Sun">Der-Shan Sun</name><affiliation wicri:level="1"><nlm:affiliation>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan; Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan. Electronic address: dssun@mail.tcu.edu.tw.</nlm:affiliation><country xml:lang="fr">Taïwan</country><wicri:regionArea>Department of Molecular Biology and Human Genetics, Tzu-Chi University, Hualien, Taiwan; Institute of Medical Sciences, Tzu-Chi University, Hualien</wicri:regionArea><wicri:noRegion>Hualien</wicri:noRegion></affiliation></author></analytic><series><title level="j">Journal of virological methods</title><idno type="eISSN">1879-0984</idno><imprint><date when="2014" type="published">2014</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antiviral Agents (isolation & purification)</term><term>Binding Sites</term><term>Cell Adhesion</term><term>Cell Line</term><term>Humans</term><term>Peptides (isolation & purification)</term><term>Protein Binding</term><term>SARS Virus (physiology)</term><term>Spike Glycoprotein, Coronavirus (metabolism)</term><term>Virus Attachment</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adhérence cellulaire</term><term>Animaux</term><term>Antiviraux (isolement et purification)</term><term>Attachement viral</term><term>Glycoprotéine de spicule des coronavirus (métabolisme)</term><term>Humains</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Peptides (isolement et purification)</term><term>Sites de fixation</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antiviral Agents</term><term>Peptides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antiviraux</term><term>Peptides</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéine de spicule des coronavirus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Binding Sites</term><term>Cell Adhesion</term><term>Cell Line</term><term>Humans</term><term>Protein Binding</term><term>Virus Attachment</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adhérence cellulaire</term><term>Animaux</term><term>Attachement viral</term><term>Humains</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Sites de fixation</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Emerging life threatening pathogens such as severe acute aspiratory syndrome-coronavirus (SARS-CoV), avian-origin influenzas H7N9, and the Middle East respiratory syndrome coronavirus (MERS-CoV) have caused a high case-fatality rate and psychological effects on society and the economy. Therefore, a simple, rapid, and safe method to investigate a therapeutic approach against these pathogens is required. In this study, a simple, quick, and safe cell adhesion inhibition assay was developed to determine the potential cellular binding site on the SARS-CoV spike protein. Various synthetic peptides covering the potential binding site helped to minimize further the binding motif to 10-25 residues. Following analyses, 2 peptides spanning the 436-445 and 437-461 amino acids of the spike protein were identified as peptide inhibitor or peptide vaccine candidates against SARS-CoV. </div></front></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><country name="Taïwan"><noRegion><name sortKey="Chang, Hsin Hou" sort="Chang, Hsin Hou" uniqKey="Chang H" first="Hsin-Hou" last="Chang">Hsin-Hou Chang</name></noRegion><name sortKey="Chen, Po Kong" sort="Chen, Po Kong" uniqKey="Chen P" first="Po-Kong" last="Chen">Po-Kong Chen</name><name sortKey="Dong, Jing Hua" sort="Dong, Jing Hua" uniqKey="Dong J" first="Jing-Hua" last="Dong">Jing-Hua Dong</name><name sortKey="Hsiao, Yu Cheng" sort="Hsiao, Yu Cheng" uniqKey="Hsiao Y" first="Yu-Cheng" last="Hsiao">Yu-Cheng Hsiao</name><name sortKey="Liao, Chih Hsien" sort="Liao, Chih Hsien" uniqKey="Liao C" first="Chih-Hsien" last="Liao">Chih-Hsien Liao</name><name sortKey="Lin, Guan Ling" sort="Lin, Guan Ling" uniqKey="Lin G" first="Guan-Ling" last="Lin">Guan-Ling Lin</name><name sortKey="Sun, Der Shan" sort="Sun, Der Shan" uniqKey="Sun D" first="Der-Shan" last="Sun">Der-Shan Sun</name><name sortKey="Wang, Chun Jen" sort="Wang, Chun Jen" uniqKey="Wang C" first="Chun-Jen" last="Wang">Chun-Jen Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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